Bénéfice d’un copolymère injectable d’acides gras dans le traitement des maladies articulaires

Résumé

MÉTHODOLOGIE

→ Une étude in vitro et in vivo a évalué les effets d’un traitement avec un copolymère d’acides gras (ara 3000 beta®) sur une inflammation des chondrocytes et des synoviocytes.

RÉSULTATS

→ Le copolymère d’acides gras réduit nettement l’inflammation dans l’articulation. In vitro, il limite la production des métalloprotéases matricielles et de l’interleukine 6, la sécrétion d’oxyde nitrique et de prostaglandine E2 et l’activation de NFκB. In vivo, l’ara 3000 beta® diminue l’œdème, l’érythème et l’ankylose.

CONCLUSION

→ Les résultats de cette étude suggèrent qu’un copolymère d’acides gras possède des propriétés anti-inflammatoires et freine la dégradation du cartilage associée aux maladies articulaires inflammatoires chroniques.

Summary

Anti-inflammatory effects of an injectable copolymer of fatty acids in joint diseases

METHODS

→ Inflammation was mimicked in vitro by treatment of human articular chondrocytes with interleukin-1β, and in synoviocytes with LPS. Effects of a treatment with a copolymer of fatty acids (ara 3000 beta®) were determined by evaluating MMP production by RT-PCR and ELISA, NO release by Griess assay, and PGE2 expression by ELISA. In addition, in vivo analysis (evolution of weight and edema) were also performed after injection of Freund adjuvant in rats.

RESULTS

→ The copolymer of fatty acids clearly reduced inflammation in joint. In vitro, it impaired IL1 stimulated-MMP production and release, as well as release of NO and PGE2 and activation of NFκB in chondrocytes. Also, in synoviocytes, it reduces the induction of MMP and IL-6. Furthermore, in vivo experiments corroborate the anti-inflammatory effects of ara 3000 beta®, with a reduction of edema, erythema and ankylosis in arthritic rats.

CONCLUSION

→ The results support the hypothesis that a copolymer of fatty acids, such as ara 3000 beta® has anti-inflammatory properties, suggesting a beneficial potential for preventing cartilage degradation associated with chronic inflammatory joint diseases.

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